Objective 3. Testing the response of Ips duplicatus adults to various combinations of aggregative synthetic pheromone with the monoterpenes alpha-pinene, limonene and myrcen



Testing the response of Ips duplicatus adults to various combinations of aggregative synthetic pheromone with monoterpene alpha-pinene, limonene and myrcene. (O3). Experiments carried out under this objective also consider the analysis of the response o the Ips duplicatus beetles, but this time using next to synthetic pheromone the volatile substances characteristic for spruce also synthetically produced (alpha-pinene, limonene, myrcen). There will be developed at least three experiments in the field. For all experiments there will be used dispensers with synthetic pheromone release fixed rate (same rate across all experiments). Experiments will differentiate by the combinations of monoterpenes used and the rates of their release. A first experiment will test, for example, the Ips typographus adults’ response to the combination of synthetic pheromone and (-)-alpha-pinene, when the release rate of monoterpenes varies. In each experiment there will be tested at least five different experiments, each variant being characterized by a specific release rate of monotetpene or combinations of monoterpenes. Variants tested will also be installed in the wing-type traps. Each experiment will be carried out in at least two areas, similar in terms of station.

As with the first goal, the catches in traps will be collected every 3-4 days. At harvest there will also be rotated dispensers. It is expected that in each experiment, the biological material is collected at least five times. Also, the dispenser’s variants will be rotated so that each version goes at least once by each trap position in the block. As with the experiments that will be developed in the first objective, the traps from the field will be installed in network of squares, with sides of 15 m, the first traps being located at a distance of at least 20 m from the forest edge.

Works laboratories necessary for developing these goals consist primarily in preparation and calibration of both pheromone dispensers and those with terpenes. For this it will be used the same procedures as those described in the first goal. Biological material collected in the field will be analyzed in the laboratory, which is sorted, identified as species and as sex.

Results expected in this case are that the response of the Ips duplicatus beetle in flight will increase in intensity together with the increasing rate of diffusion of monoterpenelor to certain, beyond which the intensity of the response will be reduced. It also is expected the response to be inhibited to certain combinations of monoterpenes shown along with synthetic pheromones and the gender ratio varies in favor of male catches in traps baited with combinations of monoterpenes.